By Eigil Kjeldsen, Steen Kølvraa (auth.), Bernd W. Rautenstrauss, Thomas Liehr (eds.)
From the reviews:
"Ever puzzled tips on how to seize your FISH … at nighttime swimming pools of mobile biology? Then … FISH know-how could be a welcome boost in your library. Thirty-six chapters on 485 pages bring together the newest protocols and history details on fluorescence in situ hybridization (or FISH) … . each bankruptcy is illustrated by means of numerous schematic drawings … . The guide is finished by means of a subject matter index … . The ebook is definitely transparent … supplying necessary protocols for either starting FISHers and for FISH experts." (Dr. Jan Roelof Van der Meer, BioWorld, factor 6, 2002)
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Extra resources for FISH Technology
Sample text
I. The karyotype of man. J Hered 43:167-172 Hulten MA, Gould CP, Goldman ASH, Waters JJ (1991) Chromosome in situ suppression hybridization in clinical cytogenetics. J Med Genet 28:577-582 Iida S, Rao PH, Butler M, Corradini P, Boccadoro M, Klein B, Chaganti RS, Dalla-Favera R (1997) Deregulation of MUM l/IRF4 by chromosomal translocation in multiple myeloma. Nat Genet 17:226-230 Kallioniemi A, Kallioniemi O-P, Sudar D, Rutovitz D, Gray JW, Waldman F, Pinkel D (1992) Comparative genomic hybridization for molecular cytogentic analysis of solid tumours.
Mutagenesis 11:139-144 Baldini A, Ward DC (1991) In situ hybridization banding of human chromosomes with Alu-PCR products: a simultaneous karyotype for gene mapping studies. Genomics 9:770-774 Barlow AL, Hulten MA (1996) Combined immunocytogenetics and molecular cytogenetic analysis of meiosis I human spermatocytes. Chromosome Res 4:562-573 Bernell P, Avidsson I, Jacobsen B, Hast R (1996) Fluorescence in situ hybridization in combination with morphology detects minimal residual disease in remission and heralds relapse in acute leukemia.
24-color karyotyping and CGH thus represents the next generation of cytogenetic techniques which, like chromosome banding, overviews the whole genome but with far higher sensitivity. The potential use of FISH techniques is so enormous that it is impossible in a comprehensive way to cover all areas, but in the following sections, some of the more recent and general applications of FISH technology will be briefly reviewed with emphasis on clinical cytogenetics and tumor cytogenetics. Molecular cytogenetics as a diagnostic tool in clinical cytogenetics and oncology The vast majority of chromosome analyses in clinical cytogenetics and in oncology are still performed as an analysis of banded metaphases.