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To calculate the required amount of a standard fragment for a gene reconstruction, use a straight edge to align the haploid genome size with the known size of standard gene fragment and read off the amount of probe DNA equivalent to one gene copy in one μ% genomic DNA, where the line intersects the right-hand bar. For the analysis of larger amounts of genomic DNA, simply multiply the result by the number of //g analyzed; similarly for multiple gene copies multiply the result by 2, 5 or 10 as appropriate for the required number of gene copies for the reconstruction.
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