Hormonally Defined Media: A Tool in Cell Biology Lectures by Gordon H. Sato (auth.), Dr. G. Fischer, Dr. R. J. Wieser

By Gordon H. Sato (auth.), Dr. G. Fischer, Dr. R. J. Wieser (eds.)

Until a few years in the past serum or crude tissue extracts have been used pre­ dominantly or solely as media supplementations for the cultivation of cells. besides the fact that, in this time proof gathered that those sup­ plements couldn't offer in an optimum manner lots of the cultivated cells with all components valuable for his or her survival, their prolifer­ ation and/or differentiation. furthermore, a number of cells couldn't be cultivated in any respect below those stipulations and infrequently the composition of the cultures replaced inside of relatively brief classes of time by means of overgrowth of in the beginning current subpopulations of these cells which develop good in those supplementations, as for instance fibroblasts. however, utilizing those supplementations (or fractions thereof), perception may be received into the various impacts of serum or tissue extract materials with re­ gard to survival, proliferation and differentiation of cells in cul­ ture. It turned noticeable from those experiments that serum or tissue extracts didn't basically provide cells with foodstuff or supplements (which at the moment are components of all simple media), but additionally with hormones in addition to growth-, differentiation-, and attachment-factors. In process time experiments have been played within which serum enriched with hormones and different development components used to be used to effectively culti­ vate these cells that could no longer live on in serum-supplemented media by myself. less than basic stipulations in an organism, despite the fact that, just a small inhabitants of cells has direct touch with serum.

Show description

Read or Download Hormonally Defined Media: A Tool in Cell Biology Lectures and Posters Presented at the First European Conference on Serum-Free Cell Culture Heidelberg, October 7–9, 1982 PDF

Similar biology books

Transgenic Crops IV (Biotechnology in Agriculture and Forestry, Vol. 59)

Genetic engineering is a robust software for crop development. Crop biotechnology sooner than 2001 was once reviewed in Transgenic plants I-III, yet contemporary advances in plant cellphone and molecular biology have caused the necessity for brand spanking new volumes. Transgenic vegetation IV bargains with cereals, greens, root vegetation, herbs and spices.

Additional info for Hormonally Defined Media: A Tool in Cell Biology Lectures and Posters Presented at the First European Conference on Serum-Free Cell Culture Heidelberg, October 7–9, 1982

Sample text

Aline) were necessary for continuous culturing of many cell lines alanine, asparagine, aspartic acid and glutamic acid could be deleted from the cell culture media without negative effects on cell proliferation. To obtain media devoid of free amino acids sera lacking free amino acids can be prepared by dialysis or ultrafiltration. However, even then it is impossible to entirely exclude the effects of amino acids because free amino acids may be released from the treated serum, probably due to detoriation of serum protein.

Ham, R. G. and W. L. McKeehan. 1978a. Development of improved media and culture conditions for clonal growth of normal diploid cells. In Vitro 14:11-22. 29 Ham, R. G. and W. L. McKeehan. 1978b. Nutritional requirements for clonal growth of nontransformed cells. In: Nutritional Requirements of Cultured Cells. (H. ). Japan ScientiflC societies Press, Tokyo, pp. 63-115. Ham, R. G. and G. L. Sattler. 1968. Clonal growth of differentiated rabbit cartilage cells. J. Cell. Physiol. 72:109-114. Ham, R.

J. and R. G. Ham. 1981. Multiplication of human diploid fibroblasts in a synthetic medium supplemented with EGF, insulin, and dexamethasone. Exp. Cell Res. 134:303-311. Investigations on the Cultivation of Insect Cell Lines in Serum-Free Media H G. Miltenburger Zoologisches Institut der Technischen Universitiit, D-6100 Darmstadt, FRG During the last two decades the sophisticated techniques of cultivating mammalian cells in vitro have been increasingly applied to the culturing of invertebrate cells.

Download PDF sample

Rated 4.40 of 5 – based on 29 votes