By J. D. Winefordner(eds.)
A multidisciplinary method of knowing the basics of mass spectrometry for bacterial analysis
From chemotaxonomy to characterization of specified proteins, identity of Microorganisms by way of Mass Spectrometry presents an outline of either well-established and state of the art mass spectrometry ideas for opting for microorganisms. an essential software for microbiologists, health and wellbeing execs, and analytical chemists, the textual content is designed to assist scientists pick out the simplest options to be used in biomedical, biochemical, pharmaceutical, and bioterror security applications.
when you consider that microbiological purposes of mass spectrometry require a uncomplicated realizing of either microbiology and analytical chemistry, the editors have integrated fabric from either disciplines in order that readers from both box will come to appreciate the mandatory rules of the opposite. that includes contributions from one of the most famous specialists in either fields, this quantity offers particular examples of basic equipment in addition to ways built within the final decade, including:
* Metastable atom bombardment pyrolysis mass spectrometry
* Matrix-assisted laser desorption/ionization mass spectrometry (MALDI)
* MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria
* High-resolution Fourier remodel mass spectrometry (FTMS)
* Electrospray ionization (ESI) mass spectrometry
identity of Microorganisms by means of Mass Spectrometry represents the main accomplished and updated paintings at the subject at the moment on hand. it really is liberally illustrated with figures and tables and covers each point of spectrometric id of microorganisms, together with experimental techniques, a number of technique of pattern training, facts research, and interpretation of advanced mass spectral data.Content:
Chapter 1 Cultural, Serological, and Genetic equipment for id of micro organism (pages 1–21): John B. Sutherland and Fatemeh Rafii
Chapter 2 Mass Spectrometry: identity and Biodetection, classes discovered and destiny advancements (pages 23–37): Alvin Fox
Chapter three An creation to MALDI?TOF MS (pages 39–60): Rohana Liyanage and Jackson O. Lay
Chapter four the advance of the Block II Chemical organic Mass Spectrometer (pages 61–89): Wayne H. Griest and Stephen A. Lammert
Chapter five approach Reproducibility and Spectral Library meeting for swift Bacterial Characterization through Metastable Atom Bombardment Pyrolysis Mass Spectrometry (pages 91–124): Jon G. Wilkes, Gary Miertschin, Todd Eschler, Les Hosey, Fatemeh Rafii, Larry speeding, Dan A. Buzatu and Michel J. Bertrand
Chapter 6 MALDI?TOF Mass Spectrometry of Intact micro organism (pages 125–152): Jackson O. Lay and Rohana Liyanage
Chapter 7 improvement of Spectral Pattern?Matching ways to Matrix?Assisted Laser Desorption/Ionization Time?of?Flight Mass Spectrometry for Bacterial id (pages 153–160): Kristin H. Jarman and Karen L. Wahl
Chapter eight reports of Malaria via Mass Spectrometry (pages 161–180): Plamen A. Demirev
Chapter nine Bacterial pressure Differentiation via Mass Spectrometry (pages 181–201): Randy J. Arnold, Jonathan A. Karty and James P. Reilly
Chapter 10 Bacterial Protein Biomarker Discovery: A centred method of constructing Molecular?Based identity platforms (pages 203–227): Tracie L. Williams, Steven R. Monday and Steven M. Musser
Chapter eleven High?Throughput Microbial Characterizations utilizing Electrospray Ionization Mass Spectrometry and Its function in sensible Genomics (pages 229–256): Seetharaman Vaidyanathan and Royston Goodacre
Chapter 12 Bioinformatics for Flexibility, Reliability, and combination research of Intact Microorganisms (pages 257–277): Catherine Fenselau and Patrick Pribil
Chapter thirteen MALDI?FTMS of Whole?Cell micro organism (pages 279–300): Jeffrey J. Jones, Michael J. Stump and Charles L. Wilkins
Chapter 14 A evaluation of Antibody trap and Bacteriophage Amplification in reference to the Direct research of Whole?Cell micro organism by means of MALDI?TOF MS (pages 301–318): Kent J. Voorhees and Jon C. Rees
Chapter 15 Discrimination and id of Microorganisms by means of Pyrolysis Mass Spectrometry: From Burning targets to Cooling Embers—A historic standpoint (pages 319–343): Eadaoin Timmins and Royston Goodacre
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Extra info for Identification of Microorganisms by Mass Spectrometry, Volume 169
Example text
20. ; Rasooly, A. Staphylococcal enterotoxins. Int. J. Food Microbiol. 2000, 61, 1–10. 21. Fahr, A. ; Menozzi, M. G. Two-center collaborative evaluation of the performance of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of Enterococcus spp. and Staphylococcus spp. J. Clin. Microbiol. 2003, 41, 1135–1142. 22. ; Neviani, E. Molecular identification and characterization of foodassociated lactobacilli. Ital. J. Food Sci. 2000, 12, 403–423.
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