By Bent Foltmann, Vibeke Barkholt Pedersen (auth.), Jordan Tang (eds.)
In the prior ten years, a couple of lawsuits of symposia at the constitution and serve as of proteolytic enzymes were pub lished. Their insurance of acid proteases has been constrained, usually end result of the loss of major new info at the constitution of those enzymes. within the final 4 years, although, the first and tertiary constructions of a couple of acid proteases were deter mined, prompting the necessity to speak about the meanings of the previous facts and the chances for brand new experimentations. It used to be for this goal that the "Conference on Acid Proteases: constitution, functionality, and Biology" used to be geared up. It came about on the collage of Oklahoma on November 21-24, 1976. This publication is a set of the most lectures added on the convention. Acid Proteases, by means of definition refers to a gaggle of proteases having an optimum pH in acidic strategies. The vintage examples are pepsin and chymosin. a few catalytic good points are patently shared by means of those proteases, such a lot particularly, their inhibition by way of pepstatin. using lively center-directed inactivators equivalent to diazoacetyl norleucine methyl ester and 1,2-epoxy-3-(p-nitrophenoxy)propane has proven that catalytic aspartyl residues are found in every one of these enzymes. those obvious cornmon good points have triggered the advice via numerous investigators to call this staff of enzymes "aspartyl proteases" or "carboxyl proteases".
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Extra resources for Acid Proteases:Structure, Function, and Biology
Sample text
The enzyme can therefore be described as an extended sheet folding into a sandwich structure in the NH 2 -terminal lobe and the barrel in the eOOHterminal lobe. There are further interactions between the bottom of the S-barrel and the bottom of the sandwich, and many large, almost certainly aromatic groups, are thrown into the regions between and within the lobes and presumably give a hydrophobic core. 48. v. v. v. circular dichroism is shown in Figure 6b. The spectrum resembles that of pepsin (21) and pepsinogen (22).
Rotation function (5 A resolution) for chymosin versus Endothia parasitica proteinase, section B = 55° Maxin~ are at a-161°, B = 560, Y = 263° and a = 140°, B = 53°, Y = 277°. Contour levels from 35 to 75 in steps of 10 (arbitrary units). CRYSTAL STRUCTURE OF E. parasitica PROTEASE 57 The diffraction intensities calculated for the molecular electron density were correlated with those observed for the chymosin crystal at 5 A resolution by means of a Fast Rotation Function program (29). 7); these are related by a rotation of 17 0 about an axis along the length of the cleft.
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