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The first reaction is nil addition of BrOII to the 5,6double bond (Fig. 4). 5-dibromo-6-hydroxy-hydrocytosine. In alkali, 5-bromo-0-hydroxy-hydrocytosine may lose HBr, forming 5-hydroxycytosine. 4. Reaction of cytosiiie with 1)romirie-water at pH 7. It remains to be established whetJherthis is due to tautomeric shift or ionization resulting from reaction a, b, or d, or t o other causes. Studies concerning the mutational specificity of these reactions are in progress. An extensive literature exists on the incorporation of Fi-deoxybromouridine into DNA and of 5-fluorouracil into RNA (1-,5).
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