Better understanding the Campylobacter conundrum by Amélie Garénaux; et al

By Amélie Garénaux; et al

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Extra resources for Better understanding the Campylobacter conundrum

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The second technique is based on evidence of an enzymatic activity especially in the electron-transport chain. In 1992, Rodriguez et al. [318] proposed a formazan salt (5-cyano-2,3-ditolyl tetrazolium chloride (CTC)), which gives a fluorescent precipitate easily detectable inviable cells. This method, combined with 4-6 diamino 2-phenylindole (DAPI), was employed by Cappelier et al. [60] to monitor VBNC cells of C. jejuni. The third most used method corresponds to the Live/Dead kit, which employs two nucleic acid stains: viable bacteria with intact membranes fluoresce green, while damaged cells fluoresce red.

Survival of Campylobacter jejuni in Unfavourable Environments: Responses to Starvation and Osmotic Stress Introduction The Gram-negative bacterium Campylobacter jejuni is a commensal in lot of animals especially in birds [250]. C. jejuni, however, is a human gastroenteritis pathogen, and could be considered responsible for as many as 400–500 million cases worldwide each year [117]. Even if intestinal tracts are the natural niche of Campylobacter jejuni, this bacterium may be isolated in other hostile environments such as air, water, and soil [51] indicating some survival capacities.

Jejuni 81-176 chromosome and plasmids were subjected to a highthroughput nucleotide sequencing to identify the relevant unique genomic features of this strain. Two sequencing runs were performed, producing 43 contigs and 60,905,794 bp of sequence (34-fold coverage). Gaps located within the LOS (56 bp) and capsule loci (1,589 bp) were closed using published sequences. The almost closed genome thus obtained is 1,594,651 bp long, with only two remaining gaps within highly repetitive regions: 32 bp within the internal sequences of rRNA operon and 111 bp within the highly conserved Cj0794 gene of NCTC 11168 [159].

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