By Alton Meister
Advances in Enzymology and similar parts of Molecular Biology is a seminal sequence within the box of biochemistry, supplying researchers entry to authoritative reports of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, supplying an unmatched view of the historic improvement of enzymology. The sequence bargains researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in complicated organic technique, and their program in either the laboratory and undefined. each one quantity within the sequence positive aspects contributions via top pioneers and investigators within the box from all over the world. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.
With its wide selection of themes and lengthy ancient pedigree, Advances in Enzymology and comparable components of Molecular Biology can be utilized not just via scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally via any scientist attracted to the invention of an enzyme, its homes, and its functions.
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23 JAMES M . MANNING ET AL. 24 TABLE VIII Apparent 5070 S u M v a l Times of Chromate-Labeled Erythrocytes Untreated and Treated with Cyanate in Vifro. 7 - - From Gillette, Manning, and Cerami (38). Cyanate has been administered to patients with sickle-cell disease at The Rockefeller University either orally, intravenously, or by carbarnylation of erythrocytes extracorporeally. Each route of administration yielded significant carbamylation of red cells, and no dcleterious effects were observed.
Some protein-protein interactions bear similarities to polyelectrolyte phenomena and therefore may also involve ionic interactions, but these will be only briefly mentioned here since they have been well reviewed in the last few years (3-5). 11. Polyelectrolyte Activations (General) A number of enzymes show an increase in activity on the addition of a polyelectrolyte. Depending on the enzyme in question, the polyelectrolyte “activator” may be a polyanion or a polycation. Thus such polyanions as polynucleotides, sulfated glycosaminoglycans, or glycoproteins may be activators of some enzymes, whereas such polycations as polylysine, polyornithine, or protaminc are activators of others.
ELBEIN, Sun Antonio, Texas CONTENTS I. Introduction I I. Polyelectrolyte Activations (General) A. Polyanion Activations B. Polycation Activations 111. Specific Interactions of Enzymes with Polynucleotides A. Enzymes that Contain Bound Polynucleotides B. Enzymes that Are Activated by, or Form Complexes with Polynucleotides IV. Inhibition of Enzymes by Polyelectrolytes V. In Viuo Effects of Polyelectrolytes A. Effects of Polyanions on Cells B. Interaction of Polyanions with Lipoproteins C. Effect of Polyanions on Ulcer Formation D.