By Cengel
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Forty-eight patients were included and 30 have currently been grafted. Characteristics of these 2 groups of patients are indicated in Table 1. Median age of tlie patients was 55 years with 12 patients aged more than 60 years. Twenty-four patients had AML while 24 had various lymphoid malignancies. Thirty patients were in complete or partial response after their first line therapy while 18 had advanced disease, 2 of them having relapsed after previous bone marrow transplantation. Mobilization, Harvest and Cryopreservation of Peripheral Blood Stem Cells Patients were treated with G-CSF (filgrastim), 5 ~g/kg/day, SC, initiated while blood counts were within normal limits after hematopoietic recovery of previous course of Table 1.
05) compared to pretreatment value. 05) compared to G-CSF treated patients. BM specimens were analyzed for the content of CD 34+ cells (Table 1). However, neither group of patients showed the increase of more immature, myeloid lineage negative CD 34+ CD 33- cells. 6 ± 3% to 35± 6%, respectively, in patients administered CSF combination (Table 2). 05 compared to baseline value) but not of BFU-E. However, this increase was significantly lower than that observed in the posttreatment samples of patients receiving IL-3 in addition to G-CSF (Table 2).
Immature CD4+CD8+ double positive (DP) T-cells are low in Class I HLA expression, and acquire a higher Class I HLA expression at final stages of maturation ( reviewed in (6)). Cells being processed through the thymic fragment in the SCID-hu BTS grafts should therefor have variable fluorescence intensities when stained with a donor Class I HLA MAb. Histogram analysis of single positive CD4+and CD8+ cells for donor Class I HLA expression demonstrates that mature T-cells in the SCID-hu BTS grafts express high levels of donor Class I HLA, immature DP T-cells however, express low to negative levels (Figure 2 and data not shown).