Rat Jugular Vain and Carotid Artery Catheterization for by Angela Heiser, J.H.K. Liu

By Angela Heiser, J.H.K. Liu

Jugular vein and carotid artery cannulations are one of the most generally used surgical procedures in learn labs around the globe. those cannulations are vitally important for proven intravenous supply of try out components and arterial blood assortment. Cannulation reduces the strain of a number of sampling as saw in organization with tail vein or orbital sinus suggestions (Ling, 2003; Flynn, 1988; Cocchetto, 1983). only a few hostile results, together with a potential upward thrust of corticosterones and a lessen in platelets, are linked to indwelling catheters other than lower than persistent stipulations (Fagin, 1983; Richman, 1980).

Typically, technicians educate those talents to incoming staff and circulation directly to graduate reports or different fields. A ensuing "oral heritage" of cannulation has been handed down from technician to technician. i've got endeavored to catch those thoughts on paper. over the years, i've got looked for and picked up a few education fabrics and courses for educating reasons. this can be my try to bring together the entire important info in a single source.

The contents of this handbook comprise cannulation approaches special in a layout of fabrics, guideline, and pictorial for every step. moreover, the handbook is rounded out with information about methods for anesthesia, pre- and submit- operatives, anatomy, bleeding suggestions utilizing catheters implanted, and rat biodata compiled from quite a few assets. The content material is punctiliously researched and pronounced in accordance with over eighty references and seven reviewers’ suggestions.

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Observe location. 46 Pictorial Materials Procedure Two straight forceps Tie off suture (1) with surgeon’s knot to secure venotomy site. Pictorial 1 Keep sutures straight to avoid confusion while tying off. Tie off suture (2) with additional throw in opposite direction. 2 Tie off suture (3) to secure vein to catheter as anchor. Three sutures provide superior security. 3 47 Materials Procedure Large Scissors Cut extra length off. Do not cut too short to avoid unraveling. Do not leave too long to avoid wicking.

It is of note to consider that polyethylene tubing contains toxicants called phthalates. These plasticizers provide characteristics of flexibility and strength but can be absorbed by the animal during longer-term studies. Studies of phthalates in rats report hyperactive thyroid gland following exposure for three months (Price, 1988). Silicon catheters Silicon (silastic®) tubing is less likely to form clots. However, the flexibility allows for twisting/crimping post-implantation and can lead to loss of compound as it is absorbed into the walls.

12 inch PE50 marked @ 24mm with marker attached to 22 1 G needle on 10 mL syringe filled with 100IU heparinized saline – clamp catheter with hemostat at unmarked end to prevent blood flow 56 Make V-cut into top of artery between 2nd and 3rd posterior sutures. Some trapped blood will “pop” out. Coat drying vessel with this blood. Thread blunt catheter into hole up to clip. Pictorial Tip: Lift up flap of “V” with straight forceps creating a “tent” to thread catheter Materials Procedure Two straight forceps Hold forcep 1 around artery & catheter tightly just posterior to V-cut so no blood will escape.

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