Radioisotopic Methods in Hydrobiology by Yuri I. Sorokin

By Yuri I. Sorokin

Aquatic environments - marine and freshwater basins - are more and more topic to anthropogenic stresses. Likewise, there's an expanding call for for higher and extra trustworthy tools for the tracking and overview of those huge and critical ecosystems and the interrelations among the actual and chemical good points of water our bodies. using radioisotopic suggestions deals a variety of methodological chances simply because all biologically energetic compounds comprise long-living radioisotopes which make the analytical box and laboratory paintings attainable with out the precautions interested in direct radiation overall healthiness hazards.

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Cleaning of 14C-carbonate preparation can also be done in Conway dishes. In this case, the sodium hydroxide solution is placed into the central cup. The batch contents of 14C-carbonate is placed on one side of the internal area of the dish, the H 2S0 4 solution on the other. The dish is hermetically closed, and the batch is mixed with H 2S0 4. The radioactivity value of 14C in the working solution (Ri) is needed for the calculation of photosynthesis rates (see Sect. 1). It should be estimated under counting conditions similar to those of the filters with phytoplankton to determine the radioactivity of 14C assimilated by planktonic algae (R;).

The container is opened and 1-2 ml of 2 % N a2C03 solution is added into it as carrier by a Pasteur pipet Technique of Measuring Phytoplankton Primary Production 29 attached to a syringe. Then the container is placed inside a small beaker or short test tube 4-S cm high. 02N KOH solution. The flask is then closed with a rubber stopper with a funnel (Fig. 1). The end of this funnel is inserted right into the opened batch container with tht: labded carbonate. The flask is then emptied down to 100-200mmHg, and drop by drop the 2% H 2S04 solution is added into the batch until the liquid completely fills the container and more than half the volume of the beaker in which it stands.

Finally, one of the basic disadvantages of this method connected with its low sensitivity is that it needs long exposure times, not less than 24 h or often more to obtain any results. This increases the errors due to the bottle effects, as after 8-10 h or exposure in the tropics, and some 12-24 h in temperate waters, the microplankton communities begin to changes. First, rapid growth of heterotrophic bacteria starts, which influences the rate of oxygen uptake, and evidently the results of gross primary production estimates calculated by the difference in oxygen content of light and dark bottles.

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