By G.E.W.; & Millar, Elaine C.P. (Editors) Wolstenholme
Content:
Chapter 1 Chairman's starting comments (page 1): A. J. P Martin
Chapter 2 normal equipment of Paper Electrophoresis with Examples of its Use in scientific and Biochemical difficulties (pages 2–21): W. Grassmann
Chapter three brief Communications: program of Paper Electrophoresis to the Differential analysis of canines illnesses (pages 22–29): J. De Wael
Chapter four review of the Albumin?Globulin Ratio of Blood Plasma or Serum by means of Paper Electrophoresis (pages 30–42): E. M. Abdel?Wahab, V.H. Rees and D. J. R. Laurence
Chapter five research of Human Haemoglobins by way of Paper Electrophoresis (pages 43–57): J. C. White, G.H. Beaven and M. Ellis
Chapter 6 A Paper Electrophoretic research of the impact of ACTH and Cortisone at the Protein?Bound Serum Polysaccharides in a few sufferers with Rheumatoid Arthritis, Ulcerative Colitis, and so on. (pages 58–78): Helge Laurell
Chapter 7 Paper Electrophoresis (pages 79–85): E. Koiw
Chapter eight Physicochemical elements and their dating to the layout of equipment (pages 86–104): Harry Svensson
Chapter nine brief Communications: The mixed impression of Evaporation and Diffusion at the Separation of Serum Proteins through Paper Electrophoresis (pages 105–131): J. De Wael
Chapter 10 research of Separated fabrics, I (pages 132–148): E. M. Crook
Chapter eleven brief Communications: quarter less than Peaks: losing Perpendiculars as opposed to Extending Curves to Baseline (pages 149–150): Hugh J. Mcdonald
Chapter 12 a few useful issues concerning the studying of the Paper Electrophoretic Strip (pages 151–159): S. Chr. Sommerfelt
Chapter thirteen research of Separated fabrics, II (pages 160–171): N.H. Martin
Chapter 14 brief Communications: Protein?Dye Interactions thought of in terms of the Estimation of Protein in Paper Electrophoresis (pages 172–182): Geoffrey Franglen
Chapter 15 a brand new method of the Staining of Lipoproteins (pages 183–196): Hugh J. Mcdonald
Chapter sixteen the way forward for the method in its program to scientific examine and regimen research (pages 197–205): E. L. Durrum
Chapter 17 brief Communications: excessive Voltage Paper Electrophoresis (pages 206–220): B. Kickhofen
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W. GRASSMANN 20 P. (1937). Actas e trabalhos Congress0 Sul-American0 de Chimica, Rio de Janeiro e Sao Paulo, 2,334. KLOBUSITZY, D. , and KONIG, P. (1939). Arch. ezp. Puth. , 192, 271. 3. , and FISCHER, E. (1948). Naturwissenschaften, 35, 2. 4. 5. 6. 7. KoNIG, 29. (1949). , 564, 152. WIELAND, TH. (1948). Angew. , 60, 313. (1950). Angew. , 62, 31. , and ENENKEL, A. J. (1950). Natumissenschaften, 37,93. DURRUM, E. L. (1950). J . Amer. chem. , 72,2943. GRASSMANN, and HANNIG, K. (1950). Natumissenschaften, 37, 496.
0 . The total protein of these samples was low (average = 6 . ) and there was evidence of heterogeneity in the data. Gilliland and Abdel-Wahab (1956) obtained A/G ratios from 36 E. M. ABDEL-WAHAB, V. H. REES& D. J. R. LAURENCE 30 contacts screened for tuberculosis and proved to be healthy. The patterns were stained with lissamine green and the average A/G ratio obtained was 1 4. No samples gave ratios less than 1 . 0. When trailing and preferential staining of the albumin are taken into account, the A/G ratio obtained by electrophoresis (R1)is related to the true value (R) by the equation (1 zkR) R1 = (1 - 8)kR where x is the fraction of albumin that trails and k is the ratio of dye binding for equal amounts of albumin and globulin.
Since then we have collected nearly twice as much data concerning cases of hepatitis, liver cirrhosis, benign and malignant tumours. These are given in Table I , whereas some typical diagrams are represented in Fig. 1. 2 + pz S y = 5 9 . 9 1 1 . 1. Paper electrophoresis diagrams of dog sera. ( a ) normal; ( b ) hepatitis; (c) liver cirrhosis; (a) malignant tumour. facing page 22 DIFFERENTIAL DIAGNOSIS OF CANINE DISEASES 23 In Table I the percentages of the protein fractions which differ significantly from the normal values are printed in italics.