By Donald Armstrong (Editor)
Read or Download Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (Methods in Molecular Biology Vol 196) PDF
Similar biology books
Transgenic Crops IV (Biotechnology in Agriculture and Forestry, Vol. 59)
Genetic engineering is a strong instrument for crop development. Crop biotechnology prior to 2001 used to be reviewed in Transgenic plants I-III, yet fresh advances in plant mobile and molecular biology have caused the necessity for brand spanking new volumes. Transgenic plants IV offers with cereals, greens, root plants, herbs and spices.
- Nosocomial Pneumonia (Lung Biology in Health and Disease)
- Biologie, Anatomie, Physiologie. Lehrbuch und Atlas mit CD-Rom.
- Weed Ecology in Natural and Agricultural Systems
- Advances in Enzymology and Related Areas of Molecular Biology (Volume 71) Volume 71 Edition by Meister, Alton published by Wiley-Interscience Hardcover
- Membrane Processes: Molecular Biology and Medical Applications
- Thin Films and Coatings in Biology
Extra resources for Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (Methods in Molecular Biology Vol 196)
Sample text
4. 5. 1 M citric acid. 2 M Na2HPO4. , Redding, CA) (see Note 1). ) (see Note 2). , St. Louis, MO) (see Note 3). 3. 1. Tissue Fixation 1. Fix tissue in a cold, freshly prepared, buffered aldehyde fixative for 1 h. 5–5% acrolein can be used (see Note 4). 2. Wash the tissue in several changes (4 × 15 min) of cold, buffer wash to removed unreacted fixative. 2. Reaction with TMB and Post Fixation with Osmium Tetroxide 1. 0. 0. It is not necessary to check the pH with a pH meter. 2. Incubate tissue at 4°C overnight in TMB solution.
3A). In the RCS rats, the degenerating photoreceptor outer segments showed strong positive staining with anti-rat αGSH-PO, DAB, and OsO4, and fluorescent granules were visible around the nuclei of the photoreceptor cells. However, the photoreceptor inner segments were not stained (Fig. 3B). 4. Transmission Electron Microscopy of Specimens Reacted with HRP-Conjugated Anti-GSH-PO, DAB, and OsO4 (see Note 4) In the Wistar rats GSH-PO was localized in the mitochondria of the photoreceptor inner segments (Fig.
Mukkassah-Kely, S. , and Montestruque, S. (1983) The influence of phospholipase A2 and glutathione peroxidase on the elimination of membrane lipid peroxides. Arch. Biochem. Biophy. 223, 441–452. 13. , and Watanabe, K. (1992) Subcellular visualization of light microscopic specimens by laser scanning microscopy and computer analysis: a new application of image analysis. J. Histochem. Cytochem. 40, 955–967. 14. Glick, B. S. (1995) Can Hsp70 proteins act as force-generating motors? Cell 80, 11–14.