Microchip-Based Assay Systems (Methods in Molecular Biology, by Pierre N. Floriano

By Pierre N. Floriano

This state-of-the-art publication explores contemporary development within the microelectronics area. It examines the consequent miniaturization of part machine gains to nanometer dimension debris, and the consequent development within the improvement and use of microchip-based concepts in best laboratories around the globe. within the 16 chapters of this quantity, top scientists current technically distinctive microchip-based assays for numerous purposes.

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4. Treating the replica and slide with an ozone cleaner is also an effective way to make the surface hydrophilic. The PDMS replica will seal the slide irreversibly after being treated by the ozone cleaner. 5. The channel surface will regain hydrophobicity quickly when exposed to the air. After the PDMS replica is taken out of HCl solution and blown dry, the PDMS replica is immediately sealed to a clean glass slide and phosphate buffer is filled in. Rapid Electrical Lysis of Bacterial Cells 33 Fig.

We further acknowledge the Department of Homeland Security Scholars and Fellows Program for their support of Brent Satterfield while conductiong this research. References 1. Mouradian, S. (2002) Lab-on-a-chip: applications in proteomics. Curr. Opin. Chem. Biol. 6(1), 51–46. 2. , and Mersal, G. (2003) Biochemical analysis with microfluidic systems. Anal. Bioanal. Chem. 377(3), 556–469. 3. Verpoorte, E. (2002) Microfluidic chips for clinical and forensic analysis. Electrophoresis 23(5), 677–712. 4.

Schematic diagram for fabrication of Si microwell array chip. Fig. 3. Schematic of the equipment for thermal oxidation of Si chip. 2. Photolithography of the Si Chip 1. All procedures must be carried out in a yellow-lighted room to prevent exposure of photoresist-coated chips. 2. The Si chip with oxidized layer is washed by soaking in RCA solution at 80°C for 20 min, and then rinsed with water. On-Chip Bioassay in 3D Microfluidic Network 43 3. After spin-drying, the chip is mounted in a spin coater.

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