Methods in Microbiology, Vol. 7, Part A by J.R. Norris, D.W. Ribbons (Eds.)

By J.R. Norris, D.W. Ribbons (Eds.)

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SNEATH C. Evaluation ofdata A taxonomic study will usually be based on the worker’s own data, and he will need to consider it critically before proceeding further. It is certainly possible to use data from monographs and the like, but this is often unsatisfactory, because results on individual strains may not be given, the internal variation of taxa may not be recorded, and the techniques may not have been adequately standardized. Sophisticated computing cannot correct for these: “rubbish in, rubbish out”.

44 P. H. A. 0. L Probits . 2(b). Relationship between % phenetic similarity ( S S M )and % DNA pairing as probits. The data are from Jones and Sneath (1970) and Sneath (1971) with the addition of new data points from Colwell (1970). The dashed lines show the expected approximate 95% confidence limits. 834. 151 is shown by the cross). 0653 (% S). Nucleic acid resemblances should be processed further by cluster analysis. One drawback is the labour of comparing all pairs of strains, and and unfortunately we do not yet have much information on how valid and reliable are clusters that are based on only a few of the possible interstrain comparisons.

Ware, G. C. (1967a). J. theor. , 17, 91-107. Ware, G. C. (1967b). Lab. , 16, 980. Williams et al. “Elliott Simulator Package”. Elliott Bros. , Boreham Wood, Herts. Appendix-An Abbreviated Flow Diagram of the Programme and Details of a Typical Procedure The function designator “actual rate” deals with the calculation of growth rate and includes the statements considering lag delay shown in Fig. 4. 0; reduction : = old delay - delay; for h: = 1 step 1 until delay- 1 do hysteresis{h} : = hysteresis {h + reduction 1); hysteresis(de1ay) : = (hysteresis{1 *2 theoretical rate)/3 ; for h: = 1 step 1 until delay do normal rate : = normal rate hysteresis {h)/delay rateratio : = normal rate/theoretical rate end else begin rateratio: = 1 ; normal rate: = theoretical rate end; + + + 28 G.

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