By Victoria E. Cotton, Eva R. Hoffmann, Mohammed F.F. Abdullah, Rhona H. Borts (auth.), Scott Keeney (eds.)
The exact chromosome dynamics of meiosis have involved scientists for good over a century, yet lately there was a proliferation of recent facts about the pairing, recombination, and segregation of chromosomes. In Meiosis, professional researchers discover fresh advances in 3 major components: quantitative genetic equipment for research of homologous recombination and chromosome pairing, improvement of direct actual assays for DNA intermediates and items of recombination, and cytological equipment for characterizing chromosome behaviors and the styles during which particular proteins go along with meiotic chromosomes. Meiosis: quantity 1, Molecular and Genetic Methods information tools for culturing and manipulating time-honored version organisms and protocols for detecting and quantifying meiotic recombination occasions via genetic and actual research of DNA-DNA or Protein-DNA interactions. equipment are supplied for reports in yeasts, vegetation, and animals, together with mouse and human. Composed within the hugely winning tools in Molecular Biology(tm) sequence structure, every one bankruptcy features a short creation, step by step tools, an inventory of priceless fabrics, and a Notes part which stocks tips about troubleshooting and warding off identified pitfalls.
Comprehensive and state-of-the-art, Meiosis: quantity 1, Molecular and Genetic Methods is a necessary guidebook, delivering up to date and demanding new protocols for the research of meiotic chromosome dynamics.
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Additional resources for Meiosis: Volume 1, Molecular and Genetic Methods
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Campbell, D. , and Lusnak, K. (1975) Mitotic chromosome loss in a disomic haploid of S. cerevisiae. Genetics 79, 383–396. Chapter 2 Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds Susan L. Elrod, Sabrina M. Chen, Katja Schwartz, and Elizabeth O. Shuster Abstract Different sporulation and pre-sporulation regimens were compared for a number of commonly used laboratory strains of S. cerevisiae to define conditions that support high-efficiency sporulation.
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