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1n: Orloff J, Berliner RW (eds) Handbook of physiology, Sect 8. Am Physiol Soc, Washington, pp 399414 26. Schneider EG, Sacktor B (1980) Sodium gradient-dependent L-glutamate transport in renal brush border membrane vesicles. Effect of an intravesicular >extravesicular potassium gradient. J BioI Chern 255:7645-7649 27. Ullrich KJ, Rumrich G, Baldamus CA (1970) Mode of urea transport across the mammalian nephron. 1n: Schmidt-Nielsen B, Kerr DWS (eds) Urea and the kidney. Excerpta Medica, Amsterdam, pp 175-184 28.
6 mmol/l. The small secretory Ac vanished when Na+ or HC03 were omitted from the perfusates or when . , unpubl. J. Ullrich References 1. Baldamus CA, Radtke HW, Rumrich G, Sauer F, Ullrich KJ (1972) Reflection coefficient and permeability of urea in the proximal convolution of the rat kidney. J Membr BioI 7: 377-390 2. Berner W, Kinne R (1976) Transport of paraaminohippuric acid by plasma membrane vesicles isolated from rat kidney cortex. Pfliigers Arch 361 :269-277 3. Blomstedt JW, Aronson PS (1980) pH gradient-stimulated transport of urate and p-aminohippurate in dog renal microvillus membrane vesicles.
The magnitude of the changes depends on the type of substrate used and on its concentration. Usually a simple Michaelis-Menten type saturation kinetic is observed with substrate specific Km values and maximal potential changes. However, peritubular application of the same substrates does not yield comparable effects. 2 mY in response to 10 mmol/l of glutamine. These values are so small that it is difficult at present to determine whether they result from the operation of a transport system similar to that in the luminal cell membrane, which might be present in the peritubular cell membrane in much lower density, or whether they result from perfusion artifacts.