By R.L.M Pierik
In Vitro tradition of upper Plants provides an up to date and vast- ranging account of the thoughts and purposes, and has essentially been written based on functional difficulties. specified awareness has been paid to the tutorial facets.
normal methodological facets are given within the first half: laboratory set-up, composition and guidance of media, sterilization of media and plant fabric, isolation and (sub)culture, mechanization, the impact of plant and environmental elements on development and improvement, the move from test-tube to soil, aids to review. The query of why in vitro tradition is practised is roofed within the moment half: embryo tradition, germination of orchid seeds, mericloning of orchids, construction of disease-free vegetation, vegetative propagation, somaclonal version, test-tube fertilization, haploids, genetic manipulation, different functions in phytopathology and plant breeding, secondary metabolites.
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Additional info for In Vitro Culture of Higher Plants
Example text
1964 Regeneration of roots and shoots on callus tissue of Populus tremuloides (Mathes). 1965 Induction of flowering in tobacco tissue in vitro (Aghion-Prat). 1965 Differentiation of tobacco plants from single isolated cells in micro-culture (Vasil and Hildebrandt). 1967 Flower induction in Lunaria annua by vernalization in vitro (Pierik). 1967 Haploid plants obtained from pollen grains of tobacco (Bourgin and Nitsch). 1969 Karyological analysis of plants regenerated from callus cultures of tobacco (Sacristan en Melchers).
As an extreme example, we can compare a test tube with a Petri dish. The test tube has a relatively large volume and a very small surface area, resulting in little aeration and drying out, and so a low chance of contamination. On the other hand, the Petri dish has a small volume and a relatively large surface area; aeration is good, drying out is far more likely and the chance of contamination is relatively high. ) than test tubes. In general, it is true that cultures in larger flasks grow and develop (propagate) better than in small flasks.
1902 First attempt at plant tissue culture (Haberlandt). 1904 First attempt at embryo culture of selected crucifers (Hiinnig). 1909 Fusion of plant protoplasts, although the products failed to survive (Kuster). 1922 Asymbiotic germination of orchid seeds in vitro (Knudson). 1922 In vitro culture of root tips (Robbins). 1925 Embryo culture applied in interspecific crosses of Linum (Laibach). 22 1929 Embryo culture of Linum to avoid cross incompatibility (Laibach). 1934 In vitro culture of the cambium tissue of a few trees and shrubs failed to be sustained since auxin had not yet been discovered (Gautheret).