Flow Cytometry in Hematopathology (1st Edition) by Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan

By Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan

This distinctive textual content deals a scientific and useful method of the research and interpretation of FCM photos. utilizing various FCM illustrations derived from genuine well-documented medical circumstances, the authors show a step by step method of optimum FCM info research on specimens suspected of harboring hematopoietic malignancies. The dialogue strikes from easy to advanced specimens, with an emphasis on visible development research. a wide selection of hematologic problems are coated, together with leukemias and lymphomas. The spouse CD-ROM with eighty specific case reports offers extra possibilities to achieve a deeper realizing of FCM info research.

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It should be noted that a small population of normal cells (mostly residual benign lymphocytes) is almost always present, even in specimens with extensive neoplastic involvement. This minor population serves as an internal control. Its location and shape on the dot plots should not be overlooked. 38 F L O W C Y T O M E T R Y I N H E M AT O P AT H O L O G Y Lineage assignment of leukemias and lymphomas is facilitated by the fact that the neoplastic cells conserve many antigens present on normal lymphoid or myeloid cells.

Low levels (below 1%) of abnormal cells can be detected by an experienced observer when the phenotype is quite specific, such as that displayed by hairy cells. In other instances, where the phenotype is not so pathognomonic, acquiring a higher number of cells will improve the sensitivity of detection. The ungated data collection ensures that no critical cells are missed. , live-gating to enrich a small population of potentially monoclonal B-cells). All other necessary gating procedures, such as gating on CD45 to identify leukemic blasts, are performed during the subsequent analysis of the originally ungated list mode data.

1, S% 2). cells from the G0 /G1 peak. If the neoplastic cells cannot be identified by a specific marker, one can assume that normal cells, in specimens other than the bone marrow, are mostly quiescent and do not contribute significantly to the cycling pool. This assumption can be applied irrespective of how close the DNA peak of the neoplastic is to that of the normal cells. The normal cells can often be separated by their light scatter characteristics. 13). This is the method of choice when the aneuploid cells are the minor component and/or the normal cells are bone marrow hematopoietic precursors, whereby the S and G2 /M signals from the tumor cells on the single parameter DNA histogram are obscured by the “noise” from the larger normal component.

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