Extracellular Nucleic Acids by Wen-Chi Chiang, Tim Tolker-Nielsen (auth.), Yo Kikuchi,

By Wen-Chi Chiang, Tim Tolker-Nielsen (auth.), Yo Kikuchi, Elena Y. Rykova (eds.)

Extracellular nucleic acids have lately emerged as vital avid gamers within the fields of biology and the scientific sciences. within the final numerous years, extracellular nucleic acids were proven to be all in favour of not just microbial evolution as genetic components but additionally to have structural roles in bacterial groups, equivalent to biofilms. Circulating DNA and RNA were present in human blood and anticipated to be priceless as non-invasive markers for the analysis of a number of illnesses. furthermore, extracellular nucleic acids have attracted realization as lively modulators of the immune method of upper organisms, together with people. This e-book covers the majority of the newly constructing fields regarding extracellular nucleic acids, together with these of simple biology, ecology and the scientific sciences, and offers readers with the newest wisdom on them.

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Gonorrhoeae also secretes chromosomal DNA. Little is known about the mechanism by which secreted DNA is produced; however, it is clear that a type IV secretion system is involved in DNA donation (Hamilton et al. 2005). Type IV secretion systems are encoded in a 57 kbp region of the gonococcal genetic islands that are present in 80% of N. gonorrhoeae strains. This secretion system is noted for its ability to secrete both DNA and proteins. Donated DNA from own and 32 K. Tani and M. Nasu Extracellular DNA Binding and Fragmentation Outer membrane Uptake into periplasma Cytoplasmic membrane Transport Gram-negative bacteria DNA processing and transfer during transformation Fig.

35 Abstract Extracellular DNA in the natural environment is a source of nutrients and gene pools for bacteria. In sediments and biofilms, gene transfer must occur among bacteria via extracellular DNA because the concentrations of both exogenous DNA and cells are high. Some bacteria actively release naked DNA or the membrane vesicle containing the DNA into the environment. Some bacteria also produce extracellular DNA by suicide and fratricide. Released DNA is used for DNA repair, transformation, and generation of genetic diversity.

35 Abstract Extracellular DNA in the natural environment is a source of nutrients and gene pools for bacteria. In sediments and biofilms, gene transfer must occur among bacteria via extracellular DNA because the concentrations of both exogenous DNA and cells are high. Some bacteria actively release naked DNA or the membrane vesicle containing the DNA into the environment. Some bacteria also produce extracellular DNA by suicide and fratricide. Released DNA is used for DNA repair, transformation, and generation of genetic diversity.

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