Clinical Biochemistry of Domestic Animals by Jiro J Kaneko

By Jiro J Kaneko

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J . " Insulin re­ sistance is found, though inconsistently, in hyperfunction of the pituitary and adrenals. " This type of response may be observed in hyperinsulinism, hypopituitarism, and hypoadrenalism and is most often employed in suspected cases of the latter two conditions. In carrying out this test, since hypoglycemia is being induced, a glucose solution should be readily available for injection. 3. Epinephrine Tolerance The response of the blood glucose level which follows the injection of epinephrine is characteristic.

BLOOD GLUCOSE 1. Methods All blood glucose methods which have been commonly employed are based on the reducing properties of glucose. These methods employ an alkaline copper solu­ tion containing cupric ( C u ^ ^ ) copper which is reduced to the cuprous ( C u + ) form. The reduced copper then reacts with a reagent, phosphomolybdate or arsenomolybdate, to form a color. Another color reaction employs the reduction o f ferricyanide to ferrocyanide to form Prussian blue. 05 ml of blood. T h e colored solutions obtained in all these methods are then compared with standard glucose solutions in a colorimeter or photometer.

T h e Nelson (1944) modification of the Somogyi method largely avoids measurement of these nonsaccharoid-reducing substances by deproteinization of the blood sample with barium and zinc solutions. For this reason, the results have often been referred to as "true" glucose values. , 1954). This method has been widely used and, unless specified, all values given in this chapter refer to "true" glucose values obtained by it. Another important consideration in the use of the Nelson-Somogyi method, particularly with ruminant bloods, is its lack of sensitivity at low concentration o f blood glucose.

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