Capillary Electrophoresis of Proteins and Peptides (Methods by Mark A. Strege, Avinash L. Lagu

By Mark A. Strege, Avinash L. Lagu

Professional educational and pharmaceutical researchers describe their most sensible capillary electrophoresis options for protein and peptide research. The authors current state-of-the-art concepts for either capillary coatings and analytic detection through laser-induced fluorescence, for the advance and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis within the evaluate of protein binding, together with using protein cost ladders. components of exact curiosity coated contain combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to accomplish proteomic reports and purposes within the box of protein-ligand binding.

Show description

Read or Download Capillary Electrophoresis of Proteins and Peptides (Methods in Molecular Biology 276) PDF

Best biology books

Transgenic Crops IV (Biotechnology in Agriculture and Forestry, Vol. 59)

Genetic engineering is a robust instrument for crop development. Crop biotechnology earlier than 2001 was once reviewed in Transgenic vegetation I-III, yet fresh advances in plant cellphone and molecular biology have brought on the necessity for brand spanking new volumes. Transgenic vegetation IV offers with cereals, greens, root plants, herbs and spices.

Additional resources for Capillary Electrophoresis of Proteins and Peptides (Methods in Molecular Biology 276)

Example text

Chem. 69, 3015–3021. 22. , Gordon, M. , and Zare, R. N. (1988) Current-monitoring method for measuring the electroosmotic flow rate in capillary zone electrophoresis. Anal. Chem. 60, 1837–1838. 23. 23 Wu, S. and Dovichi, N. J. (1989) High-sensitivity fluorescence detector for fluorescein isothiocyanate derivatives of amino acids separated by capillary electrophoresis. J. Chromatogr. 480, 141–155. 24. Zhang, J. D. thesis, Univ. Alberta, Canada. 25. 25 Cheng, Y. F. and Dovichi, N. J. (1988) Sub-attomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence.

And Suzuki O. (1988) Post-column detection for capillary zone electrophoresis. J. Chromatogr. 456, 375–381. 24. Roze D. J. and Jorgenson J. W. (1988) Post-capillary fluorescence detection in 24 capillary zone electrophoresis using o-Phthaldialdehyde. J. Chromatogr. 447, 117–131. 38 Ahmadzadeh and Krylov 25. 25 Pentoney S. , Burgi D. , and Zare R. N. (1988) On-line connector microcolumns: application to the on-column o-Ohthaldialdehyde derivatization of amino acids separated by capillary zone electrophoresis.

Totowa, NJ 39 40 Sowell et al. detection schemes use visible fluorophores, with the fluorescein–Ar-ion laser couple being one of the most popular. However, a problem often encountered with visible LIF is autofluorescence from the sample matrix. Autofluorescence arises from the intrinsic fluorescence that many biological compounds have in the visible region of the spectrum. The presence of matrix autofluorescence degrades both sensitivity and selectivity, often preventing low concentration applications that are actually the most important in bioanalytical and biomedical applications.

Download PDF sample

Rated 4.68 of 5 – based on 4 votes