By Mark A. Strege, Avinash L. Lagu
Professional educational and pharmaceutical researchers describe their most sensible capillary electrophoresis options for protein and peptide research. The authors current state-of-the-art concepts for either capillary coatings and analytic detection through laser-induced fluorescence, for the advance and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis within the evaluate of protein binding, together with using protein cost ladders. components of exact curiosity coated contain combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to accomplish proteomic reports and purposes within the box of protein-ligand binding.
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Additional resources for Capillary Electrophoresis of Proteins and Peptides (Methods in Molecular Biology 276)
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Totowa, NJ 39 40 Sowell et al. detection schemes use visible fluorophores, with the fluorescein–Ar-ion laser couple being one of the most popular. However, a problem often encountered with visible LIF is autofluorescence from the sample matrix. Autofluorescence arises from the intrinsic fluorescence that many biological compounds have in the visible region of the spectrum. The presence of matrix autofluorescence degrades both sensitivity and selectivity, often preventing low concentration applications that are actually the most important in bioanalytical and biomedical applications.