By G. Rakow (auth.), Professor Dr. Eng-Chong Pua, Professor Dr. Carl J. Douglas (eds.)
Recent advances in plant phone and molecular biology have opened new avenues for the advance of crop vegetation within the genus "Brassica" - oilseeds and greens of globally monetary value. This quantity stories advances in numerous components of "Brassica" biotechnology. It covers using rapid-cycle brassicas, tissue tradition and gene move, molecular genetics, biotic and abiotic rigidity resistance, and molecular farming. participants are world-leading foreign "Brassica" researchers. the amount is a useful reference for plant breeders, researchers and graduate scholars within the fields of plant biotechnology, agronomy, horticulture, genetics, and cellphone and molecular biology.
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2002) are constructing an integrated physical and genetic map that they expect to contain 10,000 “physical functional markers” based on A. thaliana ESTs. The intense mapping activity dedicated to this crop is expected to result in the near future in the identification of genes of economic importance to expedite its genetic improvement. 4 B. juncea Maps Sharma et al. (2002) expanded an earlier map (Sharma et al. 1994) after developing recombinant inbreds. This map contains 130 RAPD markers on 21 linkage groups.
Rapa (Teutonico and Osborn 1994), but this project used restriction fragment length polymorphisms (RFLP) that require Southern blot analysis and incubation with radioactive labeled probe. Polymerase chain reaction (PCR) can be used to detect molecular markers and is preferable for undergraduate laboratories because it is fast and does not involve radioactive materials. Therefore, ISAT students have been working to convert the RFLP markers used in the B. rapa RFLP map to PCR-based, CAPS markers (Konieczny and Ausubel 1993).
Plant Syst Evol 225:235–240 Genome Mapping and Analysis 41 Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica.