Advances in relaxation research : applications inventories by Jonathan C Smith (ed.)

By Jonathan C Smith (ed.)

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The inhibitory effect of granaticin on growth and nucleic acid synthesis can also be reversed by cystein, not by reducing agents. A second possibility to explain the inhibitory effect of quinones on nucleic acid synthesis is that the quinones interfere with the phosphorylation of the precursors. g/ml Granatlcln 0 01 02 03 % cpm/OD 4/55 165 235 1548 1423 948 102 1607 1278 891 92 1569 1280 875 87 Inhibition 10-20 40-45 90-95 Fig. 14. The effect of granaticin on the synthesis of sRNA, 16 S rRNA and 23 S rRNA in B.

Molec. BioI. 14, 179 (1965). 46. , and R. HAYES: Biochem. biophys. Res. Commun. 19, 462 (1965). 47. , and P. ) 206,480 (1965). 48. , and W. CARRIER: J. molec. BioI. 1'1, 237 (1966). 49. , and P. HOWARD-FLANDERS: Proc. nat. Acad. Sci. ) iiI, 293 (1964). 50. KOHN, K. , N. H. STEIGBIGEL, and C. L. SPEARS: Proc. nat. Acad. Sci. ) li3, 1154 (1965). 51. STRAUSS, B. , and M. RUBBINS: Biochim. biophys. ) 161, 68 (1968). 52. , and C. L. DAVISON: Biochem. biophys. Res. Commun. 1'1, 608 (1964). 53. : Z. physiol.

Other compounds exert their influence as inactivators of the template which directs the course of RNA synthesis. Finally, some inhibitors act as specific poisons of the catalytic activity of the enzymic protein itself. Since bivalent metal ions are required for the reaction, chelating agents also block RNA biosynthesis. In vivo, however, chelating agents cannot be used as specific inhibitors, since many cellular reactions are metal ion dependent and would be blocked simultan~ously. 1. Substrate Analogues A well known example of antimetabolites suppressing the RNA polymerase reaction is cordycepin triphosphate [5].

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